Journal: bioRxiv
Article Title: Discovery of metabolites produced by reactions between central carbon metabolites and cysteine that mark inflammatory macrophages
doi: 10.64898/2026.03.18.712640
Figure Lengend Snippet: A. volcano plot of discovery metabolomics comparing BMDMs that stimulated with LPS/IFNγ (at 48h post stimulation) versus unstimulated. Colored points indicate parent feature (blue circle) and 34 S isotope peak candidates (purple circle) with orange line indicated parent-isotope pair. Only parent feature that meet > 2.5-fold change and significance < 0.05 are labeled. Citrulline (green triangle) (and its isotope peaks and MS adducts) are labeled as a positive control for classical activation induced metabolites. Each point represents the mean of n = 3 biological replicates. B. volcano plot of metabolomic features in parallel cells cultured in media containing unlabeled cystine versus 3,3- 13 C 2 -L-cystine. Pairs with significant isotopic shift are colored blue and red. Pairs that match parent features in (A) are labeled with m/z values. Each point represents the mean, n = 3 biological replicates. C. Extracted ion chromatograms (EICs) for major cysteine adducts in LPS/IFNγ-stimulated BMDM (red) compared to in unstimulated BMDMs (blue). Identified molecular formulas based on isotopic distribution and exact mass are labeled. Formulas shown in red do not correspond to known mammalian metabolites. D-F. The changes in major cysteine-adducts in BMDMs over a time course of LPS/IFNγ stimulation (blue) versus IL-4/IL-13 stimulation (green). Mean +/- standard deviation, n= 3 biological replicates.
Article Snippet: For alternative activation, BMDMs were treated with with 20 ng ml −1 IL-4 (cat. no. 404-ML, R&D Systems) and 20 ng ml −1 IL-13 (cat. no. 413-ML-005, R&D Systems).
Techniques: Labeling, Positive Control, Activation Assay, Metabolomic, Cell Culture, Standard Deviation
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